Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3623723..3624402 Replicon chromosome
Accession NZ_LS999560
Organism Escherichia coli isolate EC-TO143

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag ECTO143_RS18155 Protein ID WP_000057523.1
Coordinates 3624100..3624402 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag ECTO143_RS18150 Protein ID WP_000806442.1
Coordinates 3623723..3624064 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO143_RS18140 3619967..3620899 - 933 WP_000883041.1 glutaminase A -
ECTO143_RS18145 3621161..3623665 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
ECTO143_RS18150 3623723..3624064 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
ECTO143_RS18155 3624100..3624402 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECTO143_RS18160 3624535..3625329 + 795 WP_000365147.1 TraB/GumN family protein -
ECTO143_RS18165 3625533..3626012 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
ECTO143_RS18170 3626036..3626836 + 801 WP_000439798.1 hypothetical protein -
ECTO143_RS18175 3626833..3627336 + 504 WP_000667000.1 hypothetical protein -
ECTO143_RS18180 3627374..3629026 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T293072 WP_000057523.1 NZ_LS999560:c3624402-3624100 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT293072 WP_000806442.1 NZ_LS999560:c3624064-3623723 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References