Detailed information of TA system TA029307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4662648..4663329
• Replicon: chromosome
• Accession: NC_020912
• Organism: Pseudomonas aeruginosa B136-33

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G655_RS21320
• Protein ID: WP_015503432.1
• Coordinates: 4662964..4663329 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G655_RS21315
• Protein ID: WP_003145733.1
• Coordinates: 4662648..4662971 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G655_RS21290	4658561..4659199	+	639	WP_003109444.1	hypothetical protein	-
G655_RS21295	4659442..4659777	+	336	WP_003140884.1	TM2 domain-containing protein	-
G655_RS21300	4659951..4660469	+	519	WP_012614469.1	PAAR domain-containing protein	-
G655_RS21305	4660466..4661167	+	702	WP_015503430.1	VRR-NUC domain-containing protein	-
G655_RS21310	4661184..4662272	+	1089	WP_015503431.1	DUF3396 domain-containing protein	-
G655_RS21315	4662648..4662971	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
G655_RS21320	4662964..4663329	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G655_RS21325	4663593..4663832	-	240	WP_044060930.1	hypothetical protein	-
G655_RS21330	4664039..4664311	+	273	WP_003124230.1	hypothetical protein	-
G655_RS21335	4664342..4664767	-	426	WP_003116492.1	VOC family protein	-
G655_RS21340	4664868..4665752	+	885	WP_003140892.1	LysR family transcriptional regulator	-
G655_RS21345	4665725..4666678	-	954	WP_003085661.1	LysR family transcriptional regulator	-
G655_RS21350	4666899..4667333	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4659442..4665752	6310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T29307 WP_015503432.1 NC_020912:c4663329-4662964 [Pseudomonas aeruginosa B136-33]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T29307 NC_020912:c4663329-4662964 [Pseudomonas aeruginosa B136-33]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGAGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT29307 WP_003145733.1 NC_020912:c4662971-4662648 [Pseudomonas aeruginosa B136-33]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT29307 NC_020912:c4662971-4662648 [Pseudomonas aeruginosa B136-33]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATCGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA