Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1243738..1244465 Replicon chromosome
Accession NZ_LS999206
Organism Enterobacter hormaechei isolate EC-TO80

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q486
Locus tag ECLTO80_RS06055 Protein ID WP_033486526.1
Coordinates 1244154..1244465 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q1H0
Locus tag ECLTO80_RS06050 Protein ID WP_045349893.1
Coordinates 1243738..1244157 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECLTO80_RS06040 1241568..1242689 - 1122 WP_015571201.1 glutamate--cysteine ligase -
ECLTO80_RS06045 1242795..1243685 + 891 WP_047642348.1 oxidoreductase -
ECLTO80_RS06050 1243738..1244157 - 420 WP_045349893.1 helix-turn-helix domain-containing protein Antitoxin
ECLTO80_RS06055 1244154..1244465 - 312 WP_033486526.1 type II toxin-antitoxin system HigB family toxin Toxin
ECLTO80_RS06060 1244819..1245088 - 270 WP_047717959.1 hypothetical protein -
ECLTO80_RS06065 1245284..1245886 + 603 WP_023320086.1 tyrosine-type recombinase/integrase -
ECLTO80_RS06070 1246023..1246691 + 669 WP_023296073.1 epoxyqueuosine reductase QueH -
ECLTO80_RS06075 1246924..1247109 - 186 Protein_1165 hypothetical protein -
ECLTO80_RS06080 1247400..1248281 - 882 WP_003858850.1 LysR family transcriptional regulator -
ECLTO80_RS06085 1248387..1249166 + 780 WP_026080550.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12506.30 Da        Isoelectric Point: 9.9435

>T293045 WP_033486526.1 NZ_LS999206:c1244465-1244154 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLTDYYRRTKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15375.45 Da        Isoelectric Point: 4.7132

>AT293045 WP_045349893.1 NZ_LS999206:c1244157-1243738 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENASPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q486

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q1H0

References