Detailed information of TA system TA293035

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 100805..101038
• Replicon: plasmid 2
• Accession: NZ_LS998786
• Organism: Escherichia coli isolate EC-TO75

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECTO75_RS23840
• Protein ID: WP_001312861.1
• Coordinates: 100880..101038 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	100805..100836 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTO75_RS23810	96927..97424	+	498	WP_042039995.1	single-stranded DNA-binding protein	-
ECTO75_RS23815	97492..97725	+	234	WP_000005990.1	DUF905 family protein	-
ECTO75_RS23820	97753..97950	+	198	Protein_117	hypothetical protein	-
ECTO75_RS23825	98005..98439	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
ECTO75_RS23830	98436..99155	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
ECTO75_RS24355	99167..99355	-	189	WP_001299721.1	hypothetical protein	-
-	99167..99364	+	198	NuclAT_1	-	-
-	99167..99364	+	198	NuclAT_1	-	-
-	99167..99364	+	198	NuclAT_1	-	-
-	99167..99364	+	198	NuclAT_1	-	-
ECTO75_RS23835	99412..100780	+	1369	Protein_121	IS3-like element IS150 family transposase	-
-	100805..100836	+	32	NuclAT_3	-	Antitoxin
-	100805..100836	+	32	NuclAT_3	-	Antitoxin
-	100805..100836	+	32	NuclAT_3	-	Antitoxin
-	100805..100836	+	32	NuclAT_3	-	Antitoxin
ECTO75_RS23840	100880..101038	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
ECTO75_RS23855	101959..102247	+	289	Protein_123	hypothetical protein	-
ECTO75_RS23860	102365..103188	+	824	Protein_124	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sitABCD	iucA / iucB / iucC / iucD / iutA	1..103198	103198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T293035 WP_001312861.1 NZ_LS998786:100880-101038 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 32 bp

>AT293035 NZ_LS998786:100805-100836 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin