Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1843528..1844030 Replicon chromosome
Accession NZ_LS998785
Organism Escherichia coli isolate EC-TO75

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECTO75_RS08830 Protein ID WP_000767829.1
Coordinates 1843776..1844030 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECTO75_RS08825 Protein ID WP_001259255.1
Coordinates 1843528..1843779 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO75_RS08800 1838706..1839773 - 1068 WP_032181149.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
ECTO75_RS08805 1839773..1840843 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
ECTO75_RS08810 1840840..1842144 - 1305 WP_021545648.1 histidinol dehydrogenase -
ECTO75_RS08815 1842150..1843049 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECTO75_RS08820 1843195..1843245 - 51 WP_001364200.1 his operon leader peptide -
ECTO75_RS08825 1843528..1843779 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECTO75_RS08830 1843776..1844030 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECTO75_RS08835 1844113..1844937 + 825 WP_000754758.1 SDR family oxidoreductase -
ECTO75_RS08840 1844983..1845912 + 930 WP_032181145.1 LysR family transcriptional regulator -
ECTO75_RS08845 1846127..1846189 + 63 WP_010723108.1 membrane protein YoeI -
ECTO75_RS08850 1846179..1847537 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECTO75_RS08855 1847716..1848774 + 1059 WP_000492339.1 transport protein YeeE -
ECTO75_RS08860 1848788..1849015 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T293015 WP_000767829.1 NZ_LS998785:1843776-1844030 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT293015 WP_001259255.1 NZ_LS998785:1843528-1843779 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References