Detailed information of TA system TA292980

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74102..74525
• Replicon: plasmid 3
• Accession: NZ_LS992194
• Organism: Escherichia coli isolate Escherichia coli str. TO217

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECTO217_RS28195
• Protein ID: WP_001312861.1
• Coordinates: 74102..74260 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	74304..74525 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTO217_RS28150	69187..69426	-	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-
ECTO217_RS28155	69534..70181	-	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
ECTO217_RS28160	70373..70756	-	384	WP_001151529.1	relaxosome protein TraM	-
ECTO217_RS28165	71089..71679	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
ECTO217_RS28170	71976..72797	-	822	WP_001234445.1	DUF945 domain-containing protein	-
ECTO217_RS28175	72908..73204	-	297	WP_001272251.1	hypothetical protein	-
ECTO217_RS28195	74102..74260	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	74304..74525	+	222	-	-	Antitoxin
-	74304..74528	-	225	NuclAT_0	-	-
-	74304..74528	-	225	NuclAT_0	-	-
-	74304..74528	-	225	NuclAT_0	-	-
-	74304..74528	-	225	NuclAT_0	-	-
ECTO217_RS28200	74540..75259	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
ECTO217_RS28205	75256..75690	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
ECTO217_RS28210	75759..77723	-	1965	WP_049144626.1	ParB/RepB/Spo0J family partition protein	-
ECTO217_RS28215	77784..78017	-	234	WP_000006003.1	DUF905 family protein	-
ECTO217_RS28220	78075..78560	-	486	WP_031311812.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..86367	86367

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T292980 WP_001312861.1 NZ_LS992194:c74260-74102 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T292980 NZ_LS992194:c74260-74102 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 222 bp

>AT292980 NZ_LS992194:74304-74525 [Escherichia coli]
ATGTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTTTCTGCCACACA
ACACGGTAACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGC
TGACCACACTCACTTTTCCTGAAAATAACCCGCTCATTCAGGCTGTTTACGGGTAATCTGTG

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin