Detailed information of TA system TA292972

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2551..2974
• Replicon: plasmid 3
• Accession: NZ_LS992194
• Organism: Escherichia coli isolate Escherichia coli str. TO217

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: ECTO217_RS27680
• Protein ID: WP_134163217.1
• Coordinates: 2551..2706 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	2750..2974 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTO217_RS27635	1..109	+	109	Protein_0	lytic transglycosylase	-
ECTO217_RS28425	408..1223	-	816	Protein_1	DUF932 domain-containing protein	-
ECTO217_RS27655	1352..1647	-	296	Protein_2	hypothetical protein	-
ECTO217_RS27675	2131..2309	+	179	Protein_3	pilus assembly protein	-
ECTO217_RS27680	2551..2706	-	156	WP_134163217.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2750..2974	-	225	NuclAT_1	-	Antitoxin
-	2750..2974	-	225	NuclAT_1	-	Antitoxin
-	2750..2974	-	225	NuclAT_1	-	Antitoxin
-	2750..2974	-	225	NuclAT_1	-	Antitoxin
ECTO217_RS27685	2786..2965	+	180	WP_001309233.1	hypothetical protein	-
ECTO217_RS27690	2943..3711	-	769	Protein_6	plasmid SOS inhibition protein A	-
ECTO217_RS27700	3708..4145	-	438	Protein_7	conjugation system SOS inhibitor PsiB	-
ECTO217_RS27705	4214..6179	-	1966	Protein_8	ParB/RepB/Spo0J family partition protein	-
ECTO217_RS27710	6240..6473	-	234	WP_000006003.1	DUF905 family protein	-
ECTO217_RS27715	6531..7016	-	486	WP_031311812.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..86367	86367

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 6056.41 Da
• Isoelectric Point: 10.4375

>T292972 WP_134163217.1 NZ_LS992194:c2706-2551 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMALRIR

Nucleotide

• Length: 156 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT292972 NZ_LS992194:c2974-2750 [Escherichia coli]
TCGCACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin