Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 641952..642679 Replicon chromosome
Accession NZ_LS992192
Organism Escherichia coli isolate Escherichia coli str. TO217

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ECTO217_RS03175 Protein ID WP_000550189.1
Coordinates 641952..642266 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ECTO217_RS03180 Protein ID WP_000560269.1
Coordinates 642263..642679 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO217_RS03155 638109..639095 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
ECTO217_RS03160 639174..639866 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
ECTO217_RS03165 639943..640446 - 504 WP_001295542.1 M48 family metallopeptidase -
ECTO217_RS03170 640531..641667 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ECTO217_RS03175 641952..642266 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ECTO217_RS03180 642263..642679 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ECTO217_RS03185 642724..644742 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ECTO217_RS03190 644968..647319 - 2352 WP_000695432.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T292940 WP_000550189.1 NZ_LS992192:641952-642266 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT292940 WP_000560269.1 NZ_LS992192:642263-642679 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References