Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 711307..712034 Replicon chromosome
Accession NZ_LS992190
Organism Escherichia coli isolate Escherichia coli str. TO148

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ECTO148_RS03550 Protein ID WP_000550189.1
Coordinates 711307..711621 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ECTO148_RS03555 Protein ID WP_000560269.1
Coordinates 711618..712034 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO148_RS03530 707464..708450 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
ECTO148_RS03535 708529..709221 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
ECTO148_RS03540 709298..709801 - 504 WP_001295542.1 M48 family metallopeptidase -
ECTO148_RS03545 709886..711022 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ECTO148_RS03550 711307..711621 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ECTO148_RS03555 711618..712034 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ECTO148_RS03560 712079..714097 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ECTO148_RS03565 714323..716674 - 2352 WP_000695432.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T292913 WP_000550189.1 NZ_LS992190:711307-711621 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT292913 WP_000560269.1 NZ_LS992190:711618-712034 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References