Detailed information of TA system TA292904

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35968..36394
• Replicon: plasmid 4
• Accession: NZ_LS992188
• Organism: Escherichia coli isolate Escherichia coli str. 3426

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EC3426_RS25965
• Protein ID: WP_001312861.1
• Coordinates: 35968..36126 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36170..36394 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC3426_RS25915	30991..31218	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
EC3426_RS25920	31355..32026	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
EC3426_RS25925	32220..32603	-	384	WP_001354030.1	relaxosome protein TraM	-
EC3426_RS25935	32926..33528	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
EC3426_RS25940	33825..34646	-	822	WP_001234445.1	DUF945 domain-containing protein	-
EC3426_RS25945	34757..35053	-	297	WP_001272251.1	hypothetical protein	-
EC3426_RS27165	35353..35730	+	378	Protein_42	hypothetical protein	-
EC3426_RS25965	35968..36126	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	36170..36394	-	225	NuclAT_0	-	Antitoxin
-	36170..36394	-	225	NuclAT_0	-	Antitoxin
-	36170..36394	-	225	NuclAT_0	-	Antitoxin
-	36170..36394	-	225	NuclAT_0	-	Antitoxin
EC3426_RS27050	36206..36385	+	180	WP_001309233.1	hypothetical protein	-
EC3426_RS25970	36406..37125	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
EC3426_RS25975	37122..37556	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EC3426_RS25980	37625..39648	-	2024	Protein_47	ParB/RepB/Spo0J family partition protein	-
EC3426_RS25985	39709..39942	-	234	WP_000006003.1	DUF905 family protein	-
EC3426_RS25990	40000..40527	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / blaNDM-15	-	1..96986	96986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T292904 WP_001312861.1 NZ_LS992188:c36126-35968 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT292904 NZ_LS992188:c36394-36170 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin