Detailed information of TA system TA292900

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85079..85343
• Replicon: plasmid 3
• Accession: NZ_LS992187
• Organism: Escherichia coli isolate Escherichia coli str. 3426

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: EC3426_RS25660
• Protein ID: WP_001331364.1
• Coordinates: 85191..85343 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	85079..85141 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC3426_RS25645	80318..82609	-	2292	WP_021520372.1	hypothetical protein	-
EC3426_RS25650	82602..83672	-	1071	WP_001542508.1	IncI1-type conjugal transfer protein TrbB	-
EC3426_RS25655	83691..84899	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	85079..85141	-	63	NuclAT_0	-	Antitoxin
-	85079..85141	-	63	NuclAT_0	-	Antitoxin
-	85079..85141	-	63	NuclAT_0	-	Antitoxin
-	85079..85141	-	63	NuclAT_0	-	Antitoxin
EC3426_RS25660	85191..85343	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
EC3426_RS25665	85415..85666	-	252	WP_001291964.1	hypothetical protein	-
EC3426_RS25670	85966..86262	+	297	WP_001275298.1	hypothetical protein	-
EC3426_RS27040	86327..86503	-	177	WP_001054897.1	hypothetical protein	-
EC3426_RS25675	86894..87103	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
EC3426_RS25680	87175..87837	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
EC3426_RS25685	87908..90076	-	2169	WP_000698354.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..99072	99072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T292900 WP_001331364.1 NZ_LS992187:85191-85343 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

Antitoxin

Nucleotide

• Length: 63 bp

>AT292900 NZ_LS992187:c85141-85079 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin