Detailed information of TA system TA292896

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 148257..148678
• Replicon: plasmid 2
• Accession: NZ_LS992186
• Organism: Escherichia coli isolate Escherichia coli str. 3426

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: EC3426_RS25000
• Protein ID: WP_001302184.1
• Coordinates: 148257..148415 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	148480..148678 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC3426_RS24965	143321..143536	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
EC3426_RS24970	143672..144319	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
EC3426_RS24975	144510..144893	-	384	WP_133963883.1	relaxosome protein TraM	-
EC3426_RS24980	145263..145835	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
EC3426_RS24985	146131..146952	-	822	WP_001234465.1	DUF945 domain-containing protein	-
EC3426_RS24990	147071..147358	-	288	WP_000107242.1	hypothetical protein	-
EC3426_RS27035	147383..147589	-	207	WP_000275854.1	hypothetical protein	-
EC3426_RS25000	148257..148415	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	148480..148678	-	199	NuclAT_0	-	Antitoxin
-	148480..148678	-	199	NuclAT_0	-	Antitoxin
-	148480..148678	-	199	NuclAT_0	-	Antitoxin
-	148480..148678	-	199	NuclAT_0	-	Antitoxin
EC3426_RS25005	148647..148752	-	106	Protein_162	plasmid SOS inhibition protein A	-
EC3426_RS25010	148907..149224	+	318	Protein_163	IS110 family transposase	-
EC3426_RS25015	149513..149764	-	252	Protein_164	iron-containing alcohol dehydrogenase	-
EC3426_RS25020	149849..150472	-	624	WP_000912556.1	potassium channel family protein	-
EC3426_RS25025	150488..151181	-	694	Protein_166	IS1 family transposase	-
EC3426_RS25030	151238..151342	+	105	Protein_167	thiosulfate reductase cytochrome B subunit	-
EC3426_RS25040	151588..152982	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..164477	164477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T292896 WP_001302184.1 NZ_LS992186:c148415-148257 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 199 bp

>AT292896 NZ_LS992186:c148678-148480 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin