Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4321321..4322120 Replicon chromosome
Accession NZ_LS992185
Organism Escherichia coli isolate Escherichia coli str. 3426

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag EC3426_RS21175 Protein ID WP_000347272.1
Coordinates 4321656..4322120 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EC3426_RS21170 Protein ID WP_001307405.1
Coordinates 4321321..4321656 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC3426_RS21155 4317106..4317876 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
EC3426_RS21160 4317892..4319226 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
EC3426_RS21165 4319601..4321172 + 1572 WP_001273738.1 galactarate dehydratase -
EC3426_RS21170 4321321..4321656 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EC3426_RS21175 4321656..4322120 + 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EC3426_RS21180 4322175..4322984 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
EC3426_RS21185 4323233..4324513 + 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
EC3426_RS21190 4324536..4325009 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
EC3426_RS21195 4325020..4325799 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
EC3426_RS21200 4325789..4326667 + 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
EC3426_RS21205 4326685..4327119 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4312396..4322120 9724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T292890 WP_000347272.1 NZ_LS992185:4321656-4322120 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT292890 WP_001307405.1 NZ_LS992185:4321321-4321656 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References