Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3877143..3877870 Replicon chromosome
Accession NZ_LS992185
Organism Escherichia coli isolate Escherichia coli str. 3426

Toxin (Protein)


Gene name higB Uniprot ID A0A829L2T9
Locus tag EC3426_RS19025 Protein ID WP_001521139.1
Coordinates 3877559..3877870 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EC3426_RS19020 Protein ID WP_000126294.1
Coordinates 3877143..3877562 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC3426_RS19000 3872228..3872755 - 528 WP_001078777.1 electron transport protein HydN -
EC3426_RS19005 3872904..3873914 - 1011 WP_015912547.1 DNA-binding transcriptional regulator AscG -
EC3426_RS19010 3874174..3875631 + 1458 WP_020234027.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
EC3426_RS19015 3875640..3877064 + 1425 WP_020234028.1 6-phospho-beta-glucosidase AscB -
EC3426_RS19020 3877143..3877562 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
EC3426_RS19025 3877559..3877870 - 312 WP_001521139.1 type II toxin-antitoxin system HigB family toxin Toxin
EC3426_RS19030 3878037..3878507 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
EC3426_RS19035 3878500..3878910 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
EC3426_RS19040 3878907..3879674 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
EC3426_RS19045 3879674..3880216 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
EC3426_RS19050 3880226..3881935 - 1710 WP_001521140.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12493.23 Da        Isoelectric Point: 9.4783

>T292886 WP_001521139.1 NZ_LS992185:c3877870-3877559 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT292886 WP_000126294.1 NZ_LS992185:c3877562-3877143 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L2T9

Antitoxin

Source ID Structure

References