Detailed information of TA system TA029288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 365403..366052
• Replicon: chromosome
• Accession: NC_020834
• Organism: Mannheimia haemolytica USDA-ARS-USMARC-185

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D648_RS01885
• Protein ID: WP_015484768.1
• Coordinates: 365720..366052 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D648_RS01880
• Protein ID: WP_015484769.1
• Coordinates: 365403..365711 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D648_RS01850	360674..361174	+	501	WP_006250294.1	protein-export chaperone SecB	-
D648_RS01855	361281..362291	+	1011	WP_006250293.1	NAD(P)H-dependent glycerol-3-phosphate dehydrogenase	-
D648_RS01860	362307..363122	+	816	WP_006250292.1	serine O-acetyltransferase	-
D648_RS01865	363171..363782	+	612	WP_015484064.1	autotransporter outer membrane beta-barrel domain-containing protein	-
D648_RS01870	364322..364771	-	450	WP_006252950.1	hypothetical protein	-
D648_RS01875	364764..365351	-	588	WP_006250245.1	SocA family protein	-
D648_RS01880	365403..365711	-	309	WP_015484769.1	helix-turn-helix domain-containing protein	Antitoxin
D648_RS01885	365720..366052	-	333	WP_015484768.1	hypothetical protein	Toxin
D648_RS01890	366177..366671	-	495	Protein_340	DNA primase	-
D648_RS01895	366671..366928	-	258	Protein_341	integrase arm-type DNA-binding domain-containing protein	-
D648_RS01905	367735..368694	+	960	WP_006250280.1	RNA polymerase sigma factor RpoH	-
D648_RS01915	368860..369153	+	294	WP_006253765.1	DUF3144 domain-containing protein	-
D648_RS01920	369171..369473	-	303	WP_006250278.1	BrnA antitoxin family protein	-
D648_RS01925	369445..369735	-	291	WP_006250894.1	BrnT family toxin	-
D648_RS01930	369873..370412	-	540	WP_006250276.1	HAD-IIIA family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-81)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12928.79 Da
• Isoelectric Point: 9.2356

>T29288 WP_015484768.1 NC_020834:c366052-365720 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MNLTFIELPPFERFRDENLTDEEYRAFQNELLDNPEKGDLIQGLNGLRKVRLADNKRNKGKRGGIRAIYYYFISKDQIYL
VTAYGKDKQEDLSIEQRKSLSKIIDLIKAL

Nucleotide

• Length: 333 bp

>T29288 NC_020834:c366052-365720 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGAATTTAACTTTTATTGAATTGCCGCCTTTTGAAAGGTTTAGAGATGAAAACCTAACCGATGAAGAGTATAGGGCTTT
CCAAAATGAGTTATTGGATAATCCAGAAAAAGGAGATTTAATTCAAGGCTTGAACGGATTGCGAAAAGTTAGATTAGCAG
ATAATAAGCGAAACAAGGGCAAACGTGGCGGAATAAGGGCAATTTATTACTACTTCATCAGTAAAGATCAGATTTATTTA
GTTACCGCATACGGCAAAGACAAGCAGGAAGATTTAAGCATTGAACAGCGTAAATCATTAAGCAAGATCATTGATTTAAT
AAAGGCTTTATAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11534.34 Da
• Isoelectric Point: 9.6558

>AT29288 WP_015484769.1 NC_020834:c365711-365403 [Mannheimia haemolytica USDA-ARS-USMARC-185]
MATLYEDLLEGLTAMKEHLEGKRTLRTETLLSPEPLQISADEVKAIRAKLNLSQAVFAKRLRTSVKTYQGWEQGKSTPNP
QATILLRLVEQSPQVFEQIARL

Nucleotide

• Length: 309 bp

>AT29288 NC_020834:c365711-365403 [Mannheimia haemolytica USDA-ARS-USMARC-185]
ATGGCAACGTTATACGAAGACTTATTAGAGGGTTTAACCGCAATGAAAGAGCATTTAGAGGGCAAGAGAACACTACGAAC
CGAAACCCTATTAAGCCCTGAACCGCTACAGATTTCAGCCGATGAAGTAAAAGCAATCAGAGCAAAGCTAAATCTCTCTC
AAGCGGTATTTGCCAAGCGTTTACGAACCAGCGTTAAAACCTATCAAGGGTGGGAACAAGGGAAAAGCACACCGAACCCA
CAAGCAACTATTCTTTTGCGATTAGTGGAACAATCCCCACAGGTATTTGAACAAATCGCACGTCTTTAA