Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1031927..1032621 Replicon chromosome
Accession NZ_LS992185
Organism Escherichia coli isolate Escherichia coli str. 3426

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag EC3426_RS04905 Protein ID WP_001263489.1
Coordinates 1032223..1032621 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag EC3426_RS04900 Protein ID WP_000554758.1
Coordinates 1031927..1032220 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC3426_RS04880 1027559..1028056 + 498 WP_060581685.1 REP-associated tyrosine transposase RayT -
EC3426_RS04885 1028280..1029992 - 1713 Protein_924 flagellar biosynthesis protein FlhA -
EC3426_RS04890 1029964..1030749 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
EC3426_RS04895 1030820..1031875 + 1056 WP_001226164.1 DNA polymerase IV -
EC3426_RS04900 1031927..1032220 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EC3426_RS04905 1032223..1032621 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EC3426_RS04910 1032631..1033083 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
EC3426_RS04915 1033401..1033607 + 207 Protein_930 RtcB family protein -
EC3426_RS04920 1033603..1034124 + 522 Protein_931 peptide chain release factor H -
EC3426_RS04925 1034181..1035638 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
EC3426_RS04930 1035899..1036357 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 1036953..1037033 + 81 NuclAT_12 - -
- 1036953..1037033 + 81 NuclAT_12 - -
- 1036953..1037033 + 81 NuclAT_12 - -
- 1036953..1037033 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T292874 WP_001263489.1 NZ_LS992185:1032223-1032621 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT292874 WP_000554758.1 NZ_LS992185:1031927-1032220 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References