Detailed information of TA system TA292866

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128833..129259
• Replicon: plasmid 2
• Accession: NZ_LS992184
• Organism: Citrobacter freundii isolate Citrobacter freundii str. U2785

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CFU2785_RS25810
• Protein ID: WP_001312861.1
• Coordinates: 128833..128991 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	129035..129259 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFU2785_RS25770	124214..124911	-	698	Protein_149	IS1 family transposase	-
CFU2785_RS25775	125082..125378	-	297	WP_001272251.1	hypothetical protein	-
CFU2785_RS26440	125770..125974	+	205	Protein_151	pilus protein	-
CFU2785_RS25795	126059..127672	-	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
CFU2785_RS25800	127703..128053	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
CFU2785_RS25805	128050..128475	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
CFU2785_RS25810	128833..128991	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	129035..129259	-	225	NuclAT_0	-	Antitoxin
-	129035..129259	-	225	NuclAT_0	-	Antitoxin
-	129035..129259	-	225	NuclAT_0	-	Antitoxin
-	129035..129259	-	225	NuclAT_0	-	Antitoxin
CFU2785_RS25815	129271..129990	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
CFU2785_RS25820	129987..130421	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CFU2785_RS25825	130490..132513	-	2024	Protein_158	ParB/RepB/Spo0J family partition protein	-
CFU2785_RS25830	132574..132807	-	234	WP_000006003.1	DUF905 family protein	-
CFU2785_RS25835	132865..133392	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	msr(E) / mph(E) / floR / tet(A) / blaCMY-2 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / armA	-	1..185156	185156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T292866 WP_001312861.1 NZ_LS992184:c128991-128833 [Citrobacter freundii]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT292866 NZ_LS992184:c129259-129035 [Citrobacter freundii]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin