Detailed information of TA system TA292836

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 123998..124424
• Replicon: plasmid 2
• Accession: NZ_LS992181
• Organism: Escherichia coli isolate Escherichia coli str. TO124

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECTO124_RS26425
• Protein ID: WP_001312861.1
• Coordinates: 123998..124156 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	124200..124424 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTO124_RS26390	119395..120084	-	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-
ECTO124_RS26395	120271..120654	-	384	WP_001151538.1	relaxosome protein TraM	-
ECTO124_RS26400	120975..121577	+	603	Protein_141	transglycosylase SLT domain-containing protein	-
ECTO124_RS26405	121874..122695	-	822	WP_001234445.1	DUF945 domain-containing protein	-
ECTO124_RS26410	122805..123101	-	297	WP_001545326.1	hypothetical protein	-
ECTO124_RS26420	123493..123697	+	205	Protein_144	pilus protein	-
ECTO124_RS26425	123998..124156	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	124200..124424	-	225	NuclAT_0	-	Antitoxin
-	124200..124424	-	225	NuclAT_0	-	Antitoxin
-	124200..124424	-	225	NuclAT_0	-	Antitoxin
-	124200..124424	-	225	NuclAT_0	-	Antitoxin
ECTO124_RS27640	124236..124415	+	180	WP_001309233.1	hypothetical protein	-
ECTO124_RS26430	124436..125155	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
ECTO124_RS26435	125152..125586	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
ECTO124_RS26440	125655..127619	-	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
ECTO124_RS26445	127680..127913	-	234	WP_000006003.1	DUF905 family protein	-
ECTO124_RS26450	127971..128498	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..149633	149633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T292836 WP_001312861.1 NZ_LS992181:c124156-123998 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT292836 NZ_LS992181:c124424-124200 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin