Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3691304..3691845 Replicon chromosome
Accession NZ_LS992171
Organism Escherichia coli isolate Escherichia coli str. TO73

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag ECTO73_RS18150 Protein ID WP_000615983.1
Coordinates 3691567..3691845 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag ECTO73_RS18145 Protein ID WP_000729703.1
Coordinates 3691304..3691564 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO73_RS18125 3686979..3687764 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ECTO73_RS18130 3687736..3689448 + 1713 Protein_3518 flagellar biosynthesis protein FlhA -
ECTO73_RS18135 3689672..3690169 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ECTO73_RS18140 3690345..3691094 - 750 WP_000056849.1 C40 family peptidase -
ECTO73_RS18145 3691304..3691564 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECTO73_RS18150 3691567..3691845 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECTO73_RS18155 3692001..3692741 + 741 WP_001225679.1 murein L,D-transpeptidase -
ECTO73_RS18160 3692712..3693479 - 768 WP_000333380.1 class II glutamine amidotransferase -
ECTO73_RS18165 3693685..3694263 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / rhs/PAAR / vgrG/tssI 3659293..3706814 47521


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T292777 WP_000615983.1 NZ_LS992171:3691567-3691845 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT292777 WP_000729703.1 NZ_LS992171:3691304-3691564 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References