292776

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3685107..3685801 Replicon chromosome
Accession NZ_LS992171
Organism Escherichia coli isolate Escherichia coli str. TO73

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag ECTO73_RS18110 Protein ID WP_001263489.1
Coordinates 3685107..3685505 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag ECTO73_RS18115 Protein ID WP_000554758.1
Coordinates 3685508..3685801 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3680695..3680775 - 81 NuclAT_13 - -
- 3680695..3680775 - 81 NuclAT_13 - -
- 3680695..3680775 - 81 NuclAT_13 - -
- 3680695..3680775 - 81 NuclAT_13 - -
ECTO73_RS18085 3681371..3681829 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
ECTO73_RS18090 3682090..3683547 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
ECTO73_RS18095 3683604..3684125 - 522 Protein_3511 peptide chain release factor H -
ECTO73_RS18100 3684121..3684327 - 207 Protein_3512 RtcB family protein -
ECTO73_RS18105 3684645..3685097 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
ECTO73_RS18110 3685107..3685505 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ECTO73_RS18115 3685508..3685801 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ECTO73_RS18120 3685853..3686908 - 1056 WP_001226164.1 DNA polymerase IV -
ECTO73_RS18125 3686979..3687764 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ECTO73_RS18130 3687736..3689448 + 1713 Protein_3518 flagellar biosynthesis protein FlhA -
ECTO73_RS18135 3689672..3690169 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / rhs/PAAR / vgrG/tssI 3659293..3706814 47521


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T292776 WP_001263489.1 NZ_LS992171:c3685505-3685107 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT292776 WP_000554758.1 NZ_LS992171:c3685801-3685508 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References