Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4821148..4821750 Replicon chromosome
Accession NZ_LS992168
Organism Escherichia coli isolate Escherichia coli str. TO60

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag ECTO60_RS23985 Protein ID WP_000897302.1
Coordinates 4821439..4821750 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ECTO60_RS23980 Protein ID WP_000356397.1
Coordinates 4821148..4821438 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO60_RS23940 4816455..4817384 + 930 WP_000027696.1 formate dehydrogenase accessory protein FdhE -
ECTO60_RS23945 4817566..4817808 - 243 WP_001068514.1 ribbon-helix-helix domain-containing protein -
ECTO60_RS23950 4818098..4818946 + 849 WP_001038650.1 hypothetical protein -
ECTO60_RS23955 4819262..4819711 + 450 WP_032140890.1 hypothetical protein -
ECTO60_RS23960 4819896..4820114 - 219 WP_001251293.1 ribbon-helix-helix domain-containing protein -
ECTO60_RS23970 4820511..4820789 - 279 WP_001296612.1 hypothetical protein -
ECTO60_RS23980 4821148..4821438 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
ECTO60_RS23985 4821439..4821750 - 312 WP_000897302.1 hypothetical protein Toxin
ECTO60_RS23990 4821979..4822887 + 909 WP_001385591.1 alpha/beta hydrolase -
ECTO60_RS23995 4822951..4823892 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
ECTO60_RS24000 4823937..4824374 - 438 WP_000560981.1 D-tyrosyl-tRNA(Tyr) deacylase -
ECTO60_RS24005 4824371..4825243 - 873 WP_000920762.1 virulence factor BrkB family protein -
ECTO60_RS24010 4825237..4825836 - 600 WP_001296610.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T292749 WP_000897302.1 NZ_LS992168:c4821750-4821439 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT292749 WP_000356397.1 NZ_LS992168:c4821438-4821148 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References