Detailed information of TA system TA029272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4627049..4627735
• Replicon: chromosome
• Accession: NC_020829
• Organism: Pseudomonas denitrificans ATCC 13867

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M4X1N2
• Locus tag: H681_RS20730
• Protein ID: WP_015478854.1
• Coordinates: 4627457..4627735 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H681_RS20725
• Protein ID: WP_015478853.1
• Coordinates: 4627049..4627444 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H681_RS20700	4623188..4624129	+	942	WP_015478849.1	ribose-phosphate pyrophosphokinase	-
H681_RS20705	4624242..4624856	+	615	WP_015478850.1	50S ribosomal protein L25/general stress protein Ctc	-
H681_RS20710	4624894..4625478	+	585	WP_041712180.1	aminoacyl-tRNA hydrolase	-
H681_RS20715	4625518..4626618	+	1101	WP_015478852.1	redox-regulated ATPase YchF	-
H681_RS20725	4627049..4627444	-	396	WP_015478853.1	HigA family addiction module antidote protein	Antitoxin
H681_RS20730	4627457..4627735	-	279	WP_015478854.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H681_RS20735	4627751..4628779	-	1029	WP_015478855.1	tyrosine-type recombinase/integrase	-
H681_RS20740	4628776..4630068	-	1293	WP_015478856.1	hypothetical protein	-
H681_RS20745	4630215..4631429	-	1215	WP_015478857.1	zonular occludens toxin	-
H681_RS20750	4631442..4631768	-	327	WP_015478858.1	DUF2523 domain-containing protein	-
H681_RS20755	4631768..4632106	-	339	WP_051070487.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4626828..4640251	13423

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10639.17 Da
• Isoelectric Point: 8.5577

>T29272 WP_015478854.1 NC_020829:c4627735-4627457 [Pseudomonas sp. ATCC 13867]
MILSFRCEETRSLFETGTSKRWSAILNVATRKLLMLHAATELRDMRSPPGNRLEPLQGNRAGQHSIRINDQWRICFVWTD
AGPTQVEIVDYH

Nucleotide

• Length: 279 bp

>T29272 NC_020829:c4627735-4627457 [Pseudomonas sp. ATCC 13867]
TTGATCCTGAGCTTTAGATGCGAAGAGACCCGCTCGTTGTTCGAGACGGGAACCTCCAAGCGGTGGAGTGCGATTCTCAA
CGTCGCAACCCGCAAATTGCTGATGCTGCATGCTGCCACTGAGCTTCGAGACATGCGCTCTCCACCGGGCAATCGCCTGG
AGCCATTACAGGGCAATCGAGCTGGCCAGCACAGCATCAGGATCAACGACCAGTGGCGCATCTGCTTCGTCTGGACGGAT
GCAGGCCCCACGCAGGTCGAGATAGTGGATTACCACTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14636.62 Da
• Isoelectric Point: 4.5129

>AT29272 WP_015478853.1 NC_020829:c4627444-4627049 [Pseudomonas sp. ATCC 13867]
MTTNGMRPIHPGEILREEFLQELDITPAALARALNVSAPTVNDIVRERRGITADMAIRLGRYFDTSAQFWMNLQSEYALA
TAYAASGEQIEHDIKPLVVLATIEAQDDAFLVQRVKERANEPSREIPLGDL

Nucleotide

• Length: 396 bp

>AT29272 NC_020829:c4627444-4627049 [Pseudomonas sp. ATCC 13867]
ATGACAACCAACGGTATGCGCCCCATCCATCCAGGGGAAATTCTGCGCGAAGAGTTCCTGCAGGAGCTGGACATCACTCC
GGCTGCGCTGGCTCGTGCCCTCAATGTCTCGGCACCGACCGTCAACGATATCGTCCGTGAGCGTCGCGGGATCACGGCAG
ACATGGCTATCCGCCTGGGACGCTACTTCGATACGTCCGCCCAGTTCTGGATGAACCTGCAAAGTGAATACGCCCTGGCA
ACTGCCTACGCTGCCAGCGGGGAACAGATTGAGCACGACATCAAGCCGCTGGTAGTTCTCGCGACGATCGAGGCCCAGGA
CGATGCTTTTCTCGTTCAGCGTGTGAAGGAGCGAGCCAACGAGCCATCCCGTGAGATTCCATTGGGTGACCTTTGA

Structures

Toxin

• AlphaFold DB: M4X1N2