Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3714930..3715624 Replicon chromosome
Accession NZ_LS483296
Organism Escherichia coli strain NCTC9966

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag DQM96_RS19500 Protein ID WP_001263493.1
Coordinates 3714930..3715328 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag DQM96_RS19505 Protein ID WP_000554757.1
Coordinates 3715331..3715624 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3710589..3710669 - 81 NuclAT_8 - -
- 3710589..3710669 - 81 NuclAT_8 - -
- 3710589..3710669 - 81 NuclAT_8 - -
- 3710589..3710669 - 81 NuclAT_8 - -
DQM96_RS19475 3711265..3711723 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
DQM96_RS19480 3711984..3713441 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
DQM96_RS19485 3713498..3714019 - 522 Protein_3564 peptide chain release factor H -
DQM96_RS19490 3714018..3714221 - 204 Protein_3565 RNA ligase RtcB family protein -
DQM96_RS19495 3714468..3714920 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
DQM96_RS19500 3714930..3715328 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
DQM96_RS19505 3715331..3715624 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
DQM96_RS19510 3715676..3716731 - 1056 WP_111724751.1 DNA polymerase IV -
DQM96_RS19515 3716802..3717587 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
DQM96_RS19520 3717559..3719271 + 1713 Protein_3571 flagellar biosynthesis protein FlhA -
DQM96_RS19525 3719487..3719984 - 498 WP_000006256.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T292169 WP_001263493.1 NZ_LS483296:c3715328-3714930 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT292169 WP_000554757.1 NZ_LS483296:c3715624-3715331 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References