Detailed information of TA system TA029216

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF1/-
• Location: 1988491..1988798
• Replicon: chromosome
• Accession: NC_020566
• Organism: Staphylococcus aureus subsp. aureus ST228

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: SAI5S5_RS14590
• Protein ID: WP_011447039.1
• Coordinates: 1988622..1988798 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF1
Locus tag	-
Coordinates	1988491..1988630 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAI5S5_RS09835	1984057..1984236	+	180	WP_000669791.1	hypothetical protein	-
SAI5S5_RS09845	1984547..1984807	+	261	WP_001791826.1	hypothetical protein	-
SAI5S5_RS09850	1984860..1985210	-	351	WP_000702263.1	complement inhibitor SCIN-A	-
SAI5S5_RS09855	1985721..1986056	-	336	Protein_1855	SH3 domain-containing protein	-
SAI5S5_RS09860	1986707..1987198	-	492	WP_000920038.1	staphylokinase	-
SAI5S5_RS09865	1987389..1988144	-	756	WP_000861038.1	CHAP domain-containing protein	-
SAI5S5_RS09870	1988156..1988410	-	255	WP_000611512.1	phage holin	-
SAI5S5_RS09875	1988462..1988569	+	108	WP_031762631.1	hypothetical protein	-
-	1988491..1988630	+	140	NuclAT_0	-	Antitoxin
-	1988491..1988630	+	140	NuclAT_0	-	Antitoxin
-	1988491..1988630	+	140	NuclAT_0	-	Antitoxin
-	1988491..1988630	+	140	NuclAT_0	-	Antitoxin
SAI5S5_RS14590	1988622..1988798	-	177	WP_011447039.1	putative holin-like toxin	Toxin
SAI5S5_RS09885	1988907..1989680	-	774	WP_000750412.1	staphylococcal enterotoxin type A	-
SAI5S5_RS09890	1990053..1990427	-	375	WP_000340977.1	hypothetical protein	-
SAI5S5_RS09895	1990483..1990770	-	288	WP_001262621.1	hypothetical protein	-
SAI5S5_RS09900	1990816..1990968	-	153	WP_001000058.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / sak / sea / groEL	1984860..2024391	39531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T29216 WP_011447039.1 NC_020566:c1988798-1988622 [Staphylococcus aureus subsp. aureus ST228]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T29216 NC_020566:c1988798-1988622 [Staphylococcus aureus subsp. aureus ST228]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT29216 NC_020566:1988491-1988630 [Staphylococcus aureus subsp. aureus ST228]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• PDB: 7NS1

Antitoxin