Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4142909..4143450 Replicon chromosome
Accession NZ_LR898874
Organism Escherichia coli isolate MSB1_4I-sc-2280412

Toxin (Protein)


Gene name yafQ Uniprot ID D8A3N8
Locus tag JMX41_RS20745 Protein ID WP_000615978.1
Coordinates 4143172..4143450 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag JMX41_RS20740 Protein ID WP_000729709.1
Coordinates 4142909..4143169 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX41_RS20720 4137944..4139083 + 1140 WP_000785650.1 flagellar type III secretion system protein FlhB -
JMX41_RS20725 4139067..4141160 + 2094 WP_001140819.1 flagellar biosynthesis protein FlhA -
JMX41_RS20730 4141276..4141773 - 498 WP_001570960.1 transposase -
JMX41_RS20735 4141950..4142723 - 774 WP_001570959.1 C40 family peptidase -
JMX41_RS20740 4142909..4143169 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JMX41_RS20745 4143172..4143450 + 279 WP_000615978.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JMX41_RS20750 4143606..4144346 + 741 WP_001225679.1 murein L,D-transpeptidase -
JMX41_RS20755 4144317..4145084 - 768 WP_000333380.1 class II glutamine amidotransferase -
JMX41_RS20760 4145200..4145778 - 579 WP_001570958.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.66 Da        Isoelectric Point: 10.0702

>T292080 WP_000615978.1 NZ_LR898874:4143172-4143450 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLLLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T292080 NZ_LR898874:4143172-4143450 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACTGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT292080 WP_000729709.1 NZ_LR898874:4142909-4143169 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT292080 NZ_LR898874:4142909-4143169 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACTATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G928

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References