Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2508276..2508838 Replicon chromosome
Accession NZ_LR898874
Organism Escherichia coli isolate MSB1_4I-sc-2280412

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag JMX41_RS12245 Protein ID WP_000605676.1
Coordinates 2508560..2508838 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JMX41_RS12240 Protein ID WP_000781370.1
Coordinates 2508276..2508560 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX41_RS12225 2503485..2506532 + 3048 WP_077684504.1 formate dehydrogenase-N subunit alpha -
JMX41_RS12230 2506545..2507429 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
JMX41_RS12235 2507422..2508075 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
JMX41_RS12240 2508276..2508560 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
JMX41_RS12245 2508560..2508838 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JMX41_RS12250 2509024..2510034 - 1011 WP_201555636.1 alcohol dehydrogenase AdhP -
JMX41_RS12255 2510168..2511865 - 1698 WP_000433469.1 malate dehydrogenase -
JMX41_RS12260 2512022..2512159 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JMX41_RS12265 2512261..2512476 - 216 WP_000495766.1 biofilm-dependent modulation protein -
JMX41_RS12270 2512821..2513252 + 432 WP_001571405.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T292068 WP_000605676.1 NZ_LR898874:c2508838-2508560 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT292068 WP_000781370.1 NZ_LR898874:c2508560-2508276 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References