291938

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4083353..4084047 Replicon chromosome
Accession NZ_LR890714
Organism Escherichia coli isolate MSB1_6C-sc-2280315

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JMW19_RS19490 Protein ID WP_001263491.1
Coordinates 4083353..4083751 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JMW19_RS19495 Protein ID WP_000554755.1
Coordinates 4083754..4084047 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW19_RS19460 4078522..4079766 - 1245 WP_000189541.1 esterase FrsA -
- 4079182..4079262 - 81 NuclAT_11 - -
- 4079182..4079262 - 81 NuclAT_11 - -
- 4079182..4079262 - 81 NuclAT_11 - -
- 4079182..4079262 - 81 NuclAT_11 - -
JMW19_RS19465 4079858..4080316 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JMW19_RS19470 4080577..4082034 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JMW19_RS19475 4082091..4082443 - 353 Protein_3813 peptide chain release factor H -
JMW19_RS19480 4082439..4082645 - 207 Protein_3814 RtcB family protein -
JMW19_RS19485 4082891..4083343 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
JMW19_RS19490 4083353..4083751 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JMW19_RS19495 4083754..4084047 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JMW19_RS19500 4084099..4085154 - 1056 WP_001550655.1 DNA polymerase IV -
JMW19_RS19505 4085225..4086010 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
JMW19_RS19510 4085982..4087694 + 1713 Protein_3820 flagellar biosynthesis protein FlhA -
JMW19_RS19515 4087799..4088077 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JMW19_RS19520 4088070..4088426 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4073289..4084047 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T291938 WP_001263491.1 NZ_LR890714:c4083751-4083353 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT291938 WP_000554755.1 NZ_LR890714:c4084047-4083754 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References