Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1295767..1296494 Replicon chromosome
Accession NZ_LR890714
Organism Escherichia coli isolate MSB1_6C-sc-2280315

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag JMW19_RS06210 Protein ID WP_000547555.1
Coordinates 1295767..1296078 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JMW19_RS06215 Protein ID WP_000126294.1
Coordinates 1296075..1296494 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW19_RS06185 1291702..1293411 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
JMW19_RS06190 1293421..1293963 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
JMW19_RS06195 1293963..1294730 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
JMW19_RS06200 1294727..1295137 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
JMW19_RS06205 1295130..1295600 + 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
JMW19_RS06210 1295767..1296078 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
JMW19_RS06215 1296075..1296494 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
JMW19_RS06220 1296573..1297997 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
JMW19_RS06225 1298006..1299463 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
JMW19_RS06230 1299723..1300733 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
JMW19_RS06235 1300882..1301409 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T291927 WP_000547555.1 NZ_LR890714:1295767-1296078 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT291927 WP_000126294.1 NZ_LR890714:1296075-1296494 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References