Detailed information of TA system TA291880

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 76105..76531
• Replicon: plasmid 2
• Accession: NZ_LR890694
• Organism: Escherichia coli isolate MSB1_4E-sc-2280348

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JMW23_RS25015
• Protein ID: WP_001312861.1
• Coordinates: 76105..76263 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	76307..76531 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW23_RS24980	71191..71430	-	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-
JMW23_RS24985	71538..72185	-	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
JMW23_RS24990	72377..72760	-	384	WP_001151529.1	relaxosome protein TraM	-
JMW23_RS24995	73093..73683	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
JMW23_RS25000	73980..74801	-	822	WP_001234445.1	DUF945 domain-containing protein	-
JMW23_RS25005	74912..75208	-	297	WP_001272251.1	hypothetical protein	-
JMW23_RS25010	75600..75804	+	205	Protein_89	pilus protein	-
JMW23_RS25015	76105..76263	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	76307..76531	-	225	NuclAT_0	-	Antitoxin
-	76307..76531	-	225	NuclAT_0	-	Antitoxin
-	76307..76531	-	225	NuclAT_0	-	Antitoxin
-	76307..76531	-	225	NuclAT_0	-	Antitoxin
JMW23_RS25020	76343..76522	+	180	WP_001309233.1	hypothetical protein	-
JMW23_RS25025	76543..77262	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
JMW23_RS25030	77259..77693	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JMW23_RS25035	77762..79726	-	1965	WP_117389470.1	ParB/RepB/Spo0J family partition protein	-
JMW23_RS25040	79787..80020	-	234	WP_000006003.1	DUF905 family protein	-
JMW23_RS25045	80078..80563	-	486	WP_031311812.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-27	senB	1..112822	112822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T291880 WP_001312861.1 NZ_LR890694:c76263-76105 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T291880 NZ_LR890694:c76263-76105 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT291880 NZ_LR890694:c76531-76307 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin