Detailed information of TA system TA291780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 350324..350970
• Replicon: chromosome
• Accession: NZ_LR890669
• Organism: Klebsiella pneumoniae isolate INF321-sc-2280120

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JMW44_RS01630
• Protein ID: WP_064179145.1
• Coordinates: 350324..350671 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JMW44_RS01635
• Protein ID: WP_064179144.1
• Coordinates: 350671..350970 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW44_RS01620	346250..347683	+	1434	WP_201507384.1	glycogen synthase GlgA	-
JMW44_RS01625	347701..350148	+	2448	WP_021312822.1	glycogen phosphorylase	-
JMW44_RS01630	350324..350671	+	348	WP_064179145.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JMW44_RS01635	350671..350970	+	300	WP_064179144.1	helix-turn-helix domain-containing protein	Antitoxin
JMW44_RS01640	351033..352541	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
JMW44_RS01645	352746..353075	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
JMW44_RS01650	353126..353956	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
JMW44_RS01655	354006..354764	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13535.48 Da
• Isoelectric Point: 4.9436

>T291780 WP_064179145.1 NZ_LR890669:350324-350671 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRDLKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELQVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T291780 NZ_LR890669:350324-350671 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGATTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCAGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10887.67 Da
• Isoelectric Point: 10.4022

>AT291780 WP_064179144.1 NZ_LR890669:350671-350970 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAERRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT291780 NZ_LR890669:350671-350970 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGAGACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA