Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 65660..66387 | Replicon | plasmid 3 |
| Accession | NZ_LR890659 | ||
| Organism | Serratia marcescens isolate MSB1_9C-sc-2280320 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A5Q9LNL2 |
| Locus tag | JMX15_RS27960 | Protein ID | WP_004118600.1 |
| Coordinates | 66076..66387 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | V0AHC4 |
| Locus tag | JMX15_RS27955 | Protein ID | WP_000990392.1 |
| Coordinates | 65660..66079 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JMX15_RS27930 | 61860..62564 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| JMX15_RS27935 | 62663..63019 | + | 357 | WP_077253206.1 | recombination enhancement function domain protein | - |
| JMX15_RS27940 | 63443..64102 | + | 660 | WP_000078540.1 | hypothetical protein | - |
| JMX15_RS27945 | 64099..64428 | + | 330 | WP_004118613.1 | hypothetical protein | - |
| JMX15_RS27950 | 64421..65623 | + | 1203 | WP_003031541.1 | hypothetical protein | - |
| JMX15_RS27955 | 65660..66079 | - | 420 | WP_000990392.1 | helix-turn-helix domain-containing protein | Antitoxin |
| JMX15_RS27960 | 66076..66387 | - | 312 | WP_004118600.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| JMX15_RS27965 | 66598..66978 | + | 381 | Protein_73 | gluconate permease | - |
| JMX15_RS27970 | 67028..67723 | - | 696 | WP_004118595.1 | lactate utilization protein C | - |
| JMX15_RS27975 | 67716..69143 | - | 1428 | WP_000023895.1 | iron-sulfur cluster-binding protein | - |
| JMX15_RS27980 | 69154..69822 | - | 669 | WP_101881428.1 | (Fe-S)-binding protein | - |
| JMX15_RS27985 | 69875..70843 | + | 969 | WP_074181092.1 | IS5 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | mrkA | 1..147079 | 147079 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12386.14 Da Isoelectric Point: 10.0935
>T291758 WP_004118600.1 NZ_LR890659:c66387-66076 [Serratia marcescens]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
Download Length: 312 bp
Antitoxin
Download Length: 140 a.a. Molecular weight: 15534.71 Da Isoelectric Point: 4.4702
>AT291758 WP_000990392.1 NZ_LR890659:c66079-65660 [Serratia marcescens]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
Download Length: 420 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Q9LNL2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AHC4 |