Detailed information of TA system TA291723

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 6036..6462
• Replicon: plasmid 2
• Accession: NZ_LR890652
• Organism: Escherichia coli isolate MINF_2E-sc-2280463

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: JMW95_RS23970
• Protein ID: WP_001302184.1
• Coordinates: 6036..6194 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	6238..6462 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW95_RS23940	1209..1604	-	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-
JMW95_RS23945	1703..2392	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
JMW95_RS23950	2579..2962	-	384	WP_001151524.1	relaxosome protein TraM	-
JMW95_RS23955	3283..3885	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
JMW95_RS23960	4182..5003	-	822	WP_001234487.1	DUF945 domain-containing protein	-
JMW95_RS23965	5114..5410	-	297	WP_001272245.1	hypothetical protein	-
JMW95_RS23970	6036..6194	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	6238..6462	-	225	NuclAT_0	-	Antitoxin
-	6238..6462	-	225	NuclAT_0	-	Antitoxin
-	6238..6462	-	225	NuclAT_0	-	Antitoxin
-	6238..6462	-	225	NuclAT_0	-	Antitoxin
JMW95_RS23975	6474..7193	-	720	WP_001276236.1	plasmid SOS inhibition protein A	-
JMW95_RS23980	7190..7624	-	435	WP_000845962.1	conjugation system SOS inhibitor PsiB	-
JMW95_RS23985	7693..9657	-	1965	WP_068862930.1	ParB/RepB/Spo0J family partition protein	-
JMW95_RS23990	9721..9954	-	234	WP_000005988.1	DUF905 family protein	-
JMW95_RS23995	9917..10549	-	633	WP_039023645.1	single-stranded DNA-binding protein	-
JMW95_RS24000	10850..11281	+	432	WP_077250869.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaCTX-M-14	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..207369	207369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T291723 WP_001302184.1 NZ_LR890652:c6194-6036 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT291723 NZ_LR890652:c6462-6238 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin