Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3717196..3717890 Replicon chromosome
Accession NZ_LR890603
Organism Escherichia coli isolate MSB1_8G-sc-2280388

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag JMW40_RS17765 Protein ID WP_001263489.1
Coordinates 3717196..3717594 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JMW40_RS17770 Protein ID WP_000554758.1
Coordinates 3717597..3717890 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3712784..3712864 - 81 NuclAT_12 - -
- 3712784..3712864 - 81 NuclAT_12 - -
- 3712784..3712864 - 81 NuclAT_12 - -
- 3712784..3712864 - 81 NuclAT_12 - -
JMW40_RS17740 3713460..3713918 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JMW40_RS17745 3714179..3715636 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
JMW40_RS17750 3715693..3716214 - 522 Protein_3469 peptide chain release factor H -
JMW40_RS17755 3716210..3716416 - 207 Protein_3470 RtcB family protein -
JMW40_RS17760 3716734..3717186 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
JMW40_RS17765 3717196..3717594 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JMW40_RS17770 3717597..3717890 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JMW40_RS17775 3717942..3718997 - 1056 WP_001226164.1 DNA polymerase IV -
JMW40_RS17780 3719068..3719853 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JMW40_RS17785 3719825..3721537 + 1713 Protein_3476 flagellar biosynthesis protein FlhA -
JMW40_RS17790 3721761..3722258 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T291526 WP_001263489.1 NZ_LR890603:c3717594-3717196 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT291526 WP_000554758.1 NZ_LR890603:c3717890-3717597 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References