Detailed information of TA system TA291414

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 120647..121073
• Replicon: plasmid 2
• Accession: NZ_LR890563
• Organism: Klebsiella pneumoniae isolate INF247-sc-2280148

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JMW77_RS26155
• Protein ID: WP_001312861.1
• Coordinates: 120647..120805 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	120849..121073 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW77_RS26120	115670..115897	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
JMW77_RS26125	116034..116705	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
JMW77_RS26130	116899..117282	-	384	WP_001354030.1	relaxosome protein TraM	-
JMW77_RS26135	117605..118207	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
JMW77_RS26140	118504..119325	-	822	WP_001234445.1	DUF945 domain-containing protein	-
JMW77_RS26145	119436..119732	-	297	WP_001272251.1	hypothetical protein	-
JMW77_RS26150	120032..120409	+	378	Protein_139	hypothetical protein	-
JMW77_RS26155	120647..120805	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	120849..121073	-	225	NuclAT_0	-	Antitoxin
-	120849..121073	-	225	NuclAT_0	-	Antitoxin
-	120849..121073	-	225	NuclAT_0	-	Antitoxin
-	120849..121073	-	225	NuclAT_0	-	Antitoxin
JMW77_RS26160	121085..121804	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
JMW77_RS26165	121801..122235	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JMW77_RS26170	122304..124327	-	2024	Protein_143	ParB/RepB/Spo0J family partition protein	-
JMW77_RS26175	124388..124621	-	234	WP_000006003.1	DUF905 family protein	-
JMW77_RS26180	124679..125206	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / aadA5 / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	senB	1..159592	159592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T291414 WP_001312861.1 NZ_LR890563:c120805-120647 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT291414 NZ_LR890563:c121073-120849 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin