Detailed information of TA system TA291327

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 98219..98548
• Replicon: plasmid 2
• Accession: NZ_LR890537
• Organism: Escherichia coli isolate MSB1_8B-sc-2280300

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JMX06_RS25990
• Protein ID: WP_001312861.1
• Coordinates: 98219..98377 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	98421..98548 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMX06_RS25965	93727..94548	-	822	WP_001234469.1	DUF945 domain-containing protein	-
JMX06_RS25970	94667..94954	-	288	WP_000107535.1	hypothetical protein	-
JMX06_RS25975	94979..95185	-	207	WP_000275859.1	hypothetical protein	-
JMX06_RS25980	95098..95433	-	336	WP_013023876.1	hypothetical protein	-
JMX06_RS25985	96157..97824	+	1668	WP_001091724.1	group II intron reverse transcriptase/maturase	-
JMX06_RS25990	98219..98377	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	98421..98548	-	128	NuclAT_0	-	Antitoxin
-	98421..98548	-	128	NuclAT_0	-	Antitoxin
-	98421..98548	-	128	NuclAT_0	-	Antitoxin
-	98421..98548	-	128	NuclAT_0	-	Antitoxin
-	99990..100092	-	103	NuclAT_1	-	-
-	99990..100092	-	103	NuclAT_1	-	-
-	99990..100092	-	103	NuclAT_1	-	-
-	99990..100092	-	103	NuclAT_1	-	-
JMX06_RS26000	100104..100823	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
JMX06_RS26005	100820..101254	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
JMX06_RS26010	101309..103267	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / qepA4 / catA1 / tet(B) / sitABCD	iucA / iucB / iucC / iucD / iutA	1..158162	158162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T291327 WP_001312861.1 NZ_LR890537:c98377-98219 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 128 bp

>AT291327 NZ_LR890537:c98548-98421 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin