Detailed information of TA system TA029124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3852601..3853191
• Replicon: chromosome
• Accession: NC_020541
• Organism: Rhodanobacter denitrificans

Toxin (Protein)

• Gene name: graT
• Uniprot ID: I4WLY1
• Locus tag: R2APBS1_RS17595
• Protein ID: WP_007512781.1
• Coordinates: 3852910..3853191 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: R2APBS1_RS17590
• Protein ID: WP_007512782.1
• Coordinates: 3852601..3852900 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R2APBS1_RS17580	3848425..3848997	+	573	WP_157769821.1	hypothetical protein	-
R2APBS1_RS19830	3849090..3849248	-	159	Protein_3550	glycerophosphodiester phosphodiesterase	-
R2APBS1_RS17585	3849311..3852547	-	3237	WP_015448949.1	type I restriction endonuclease subunit R	-
R2APBS1_RS17590	3852601..3852900	-	300	WP_007512782.1	HigA family addiction module antidote protein	Antitoxin
R2APBS1_RS17595	3852910..3853191	-	282	WP_007512781.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
R2APBS1_RS17600	3853271..3854515	-	1245	WP_041677105.1	restriction endonuclease subunit S	-
R2APBS1_RS17605	3854521..3856596	-	2076	Protein_3555	SAM-dependent DNA methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-95)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10798.46 Da
• Isoelectric Point: 10.3017

>T29124 WP_007512781.1 NC_020541:c3853191-3852910 [Rhodanobacter denitrificans]
MTIQSFRCKDTQALFERRRVRRFLVIEAAARRKLELLNAATQLDYLRVPPGNRLEALAGDRKGQHSIRVNDQFRLCFVWT
ADGPGLVEIVDYH

Nucleotide

• Length: 282 bp

>T29124 NC_020541:c3853191-3852910 [Rhodanobacter denitrificans]
ATGACCATCCAGTCGTTCCGCTGCAAGGATACGCAGGCGCTGTTCGAGCGACGCCGGGTGCGGCGCTTTCTGGTGATCGA
GGCGGCGGCGCGGCGCAAGCTGGAGCTGTTGAATGCGGCGACCCAACTGGACTATCTGCGGGTGCCGCCCGGCAATCGAC
TGGAAGCCTTGGCCGGCGATCGCAAGGGTCAGCACAGCATTCGTGTGAATGACCAGTTCCGGCTGTGCTTCGTGTGGACC
GCCGACGGCCCGGGCCTGGTCGAGATCGTCGATTATCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.79 Da
• Isoelectric Point: 7.9969

>AT29124 WP_007512782.1 NC_020541:c3852900-3852601 [Rhodanobacter denitrificans]
MPRKLPPIHPGEILREEYMLPLELSSNALARALEVTPARINEIVRERRGISADTALRLARYFGNDAQSWLNLQQRYDIEC
AQDSIGGALRKITPYQAAA

Nucleotide

• Length: 300 bp

>AT29124 NC_020541:c3852900-3852601 [Rhodanobacter denitrificans]
ATGCCCCGCAAACTGCCACCCATCCACCCCGGCGAGATCCTGCGCGAGGAATACATGCTGCCGCTGGAGCTGTCGTCCAA
CGCGCTGGCCCGCGCCCTCGAAGTCACGCCTGCACGTATCAACGAGATCGTGCGCGAGCGGCGCGGCATCAGCGCCGATA
CCGCGCTGCGGCTGGCGCGCTATTTCGGCAATGACGCGCAGAGCTGGCTGAACCTGCAGCAGCGCTACGACATCGAGTGC
GCGCAGGACAGCATCGGCGGCGCACTGAGGAAAATCACGCCCTATCAGGCAGCCGCCTAG

Structures

Toxin

• AlphaFold DB: I4WLY1