Detailed information of TA system TA029123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1378339..1378946
• Replicon: chromosome
• Accession: NC_020541
• Organism: Rhodanobacter denitrificans

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I4WTS9
• Locus tag: R2APBS1_RS06155
• Protein ID: WP_007510671.1
• Coordinates: 1378339..1378653 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: R2APBS1_RS06160
• Protein ID: WP_015447258.1
• Coordinates: 1378668..1378946 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R2APBS1_RS06150	1374136..1377867	+	3732	WP_007510668.1	diguanylate cyclase	-
R2APBS1_RS06155	1378339..1378653	+	315	WP_007510671.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
R2APBS1_RS06160	1378668..1378946	+	279	WP_015447258.1	helix-turn-helix domain-containing protein	Antitoxin
R2APBS1_RS06165	1379002..1379406	-	405	WP_015447259.1	Arc family DNA-binding protein	-
R2APBS1_RS06170	1379522..1379884	+	363	WP_015447260.1	hypothetical protein	-
R2APBS1_RS19265	1379949..1380713	+	765	WP_015447261.1	hypothetical protein	-
R2APBS1_RS06185	1381008..1381349	+	342	WP_007512800.1	lipid-A-disaccharide synthase N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11641.42 Da
• Isoelectric Point: 9.8349

>T29123 WP_007510671.1 NC_020541:1378339-1378653 [Rhodanobacter denitrificans]
MIFVETPIFTAQIKDLLSDDDYGAFQRYLAENPEAGDVIKETGGLRKIRVAAKGHGKSGGARVIYYYFVSASRIAMVFAY
PKNVQENLTPAQKAALKKVIEQWR

Nucleotide

• Length: 315 bp

>T29123 NC_020541:1378339-1378653 [Rhodanobacter denitrificans]
ATGATCTTTGTTGAGACGCCAATCTTCACTGCCCAGATCAAGGATCTGCTGAGCGATGATGATTACGGGGCGTTCCAGCG
CTATCTAGCCGAGAATCCGGAGGCTGGCGACGTCATCAAGGAAACCGGCGGACTACGCAAGATCCGCGTGGCGGCGAAGG
GTCACGGCAAGAGCGGCGGCGCGCGAGTGATCTACTACTACTTCGTTTCTGCCTCGCGTATCGCCATGGTTTTCGCCTAT
CCGAAGAACGTACAAGAAAATCTGACGCCTGCGCAAAAAGCAGCACTAAAGAAAGTCATAGAGCAGTGGAGATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10263.75 Da
• Isoelectric Point: 7.2060

>AT29123 WP_015447258.1 NC_020541:1378668-1378946 [Rhodanobacter denitrificans]
MFDNLMESVTQMDEIVRGERQPSREVHIDALAVKEIRRATGLSQAKFAGIVDVQVGTLRNWEQGRREPTGPAKALLRAIS
KDPKHVLEALAD

Nucleotide

• Length: 279 bp

>AT29123 NC_020541:1378668-1378946 [Rhodanobacter denitrificans]
ATGTTCGACAACCTGATGGAAAGCGTGACCCAGATGGACGAGATTGTCCGGGGTGAGCGCCAGCCGTCGCGCGAGGTCCA
TATCGACGCGCTGGCCGTCAAGGAGATCCGCCGCGCCACTGGACTGTCACAGGCCAAGTTCGCCGGCATCGTCGACGTGC
AGGTCGGCACTCTTCGCAACTGGGAGCAGGGCAGGCGTGAACCCACTGGCCCAGCCAAGGCGCTACTGCGCGCGATCAGC
AAGGACCCCAAGCACGTATTGGAGGCGTTGGCCGACTGA

Structures

Toxin

• AlphaFold DB: A0A154QK28