Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2025461..2026002 Replicon chromosome
Accession NZ_LR890508
Organism Escherichia coli isolate MSB1_9I-sc-2280417

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag JMW57_RS09935 Protein ID WP_000615983.1
Coordinates 2025724..2026002 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag JMW57_RS09930 Protein ID WP_000729703.1
Coordinates 2025461..2025721 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW57_RS09910 2021136..2021921 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
JMW57_RS09915 2021893..2023605 + 1713 Protein_1943 flagellar biosynthesis protein FlhA -
JMW57_RS09920 2023829..2024326 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JMW57_RS09925 2024502..2025251 - 750 WP_024192679.1 C40 family peptidase -
JMW57_RS09930 2025461..2025721 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JMW57_RS09935 2025724..2026002 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JMW57_RS09940 2026158..2026898 + 741 WP_001225679.1 murein L,D-transpeptidase -
JMW57_RS09945 2026869..2027636 - 768 WP_000333380.1 class II glutamine amidotransferase -
JMW57_RS09950 2027842..2028420 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 1979448..2042931 63483


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T291229 WP_000615983.1 NZ_LR890508:2025724-2026002 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT291229 WP_000729703.1 NZ_LR890508:2025461-2025721 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References