Detailed information of TA system TA029122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 710647..711246
• Replicon: chromosome
• Accession: NC_020541
• Organism: Rhodanobacter denitrificans

Toxin (Protein)

• Gene name: graT
• Uniprot ID: I4WT83
• Locus tag: R2APBS1_RS03095
• Protein ID: WP_007510748.1
• Coordinates: 710647..710928 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: R2APBS1_RS03100
• Protein ID: WP_007510746.1
• Coordinates: 710941..711246 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R2APBS1_RS03085	705987..707345	-	1359	Protein_622	APC family permease	-
R2APBS1_RS03090	707622..710561	-	2940	WP_015446840.1	DEAD/DEAH box helicase family protein	-
R2APBS1_RS03095	710647..710928	+	282	WP_007510748.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
R2APBS1_RS03100	710941..711246	+	306	WP_007510746.1	HigA family addiction module antidote protein	Antitoxin
R2APBS1_RS03105	711384..713342	-	1959	WP_015446841.1	site-specific DNA-methyltransferase	-
R2APBS1_RS03110	713420..713848	+	429	WP_015446842.1	hypothetical protein	-
R2APBS1_RS03115	713977..715359	-	1383	WP_015446843.1	L-serine ammonia-lyase	-
R2APBS1_RS03120	715435..715665	+	231	WP_015446844.1	glutaredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (11-87)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10873.31 Da
• Isoelectric Point: 7.8618

>T29122 WP_007510748.1 NC_020541:710647-710928 [Rhodanobacter denitrificans]
VIRSFADKETARLSATGTSRRFPPDIVRWALSRLKYLHLATCPEDLREPFSNRLEMLSGNRAGQWSIRINDQWRLCFRFE
GGDAFDVEIVDYH

Nucleotide

• Length: 282 bp

>T29122 NC_020541:710647-710928 [Rhodanobacter denitrificans]
GTGATAAGGAGCTTCGCTGACAAGGAAACCGCGCGACTGTCCGCGACCGGAACAAGCCGGCGGTTTCCGCCGGACATCGT
GCGGTGGGCGCTGAGTCGTCTGAAATATTTGCATCTCGCCACCTGCCCCGAAGATCTGCGCGAGCCGTTTTCCAATCGCC
TGGAAATGTTGAGCGGCAATCGCGCAGGTCAATGGAGTATCCGCATCAACGATCAGTGGCGGCTGTGCTTCCGTTTCGAG
GGCGGCGATGCCTTCGACGTCGAGATCGTCGACTACCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11196.00 Da
• Isoelectric Point: 7.3200

>AT29122 WP_007510746.1 NC_020541:710941-711246 [Rhodanobacter denitrificans]
MSIPATTNGLPPIHPGELLEEILSDHGMSQAAFARAIGVSPMRISHVIREERPVTAELALRFGRAFGQSPQFWMNMQTDY
DLKIAERELKASLRKVLPLAA

Nucleotide

• Length: 306 bp

>AT29122 NC_020541:710941-711246 [Rhodanobacter denitrificans]
ATGTCCATCCCCGCCACCACGAACGGCCTGCCGCCCATTCATCCGGGGGAGCTCCTGGAAGAAATCCTGTCCGACCACGG
CATGAGCCAGGCGGCATTTGCCCGGGCGATCGGCGTTTCGCCGATGCGTATTTCGCATGTCATCCGCGAGGAGCGCCCCG
TCACGGCCGAACTTGCCCTGCGCTTTGGCCGCGCCTTCGGCCAGAGCCCGCAGTTCTGGATGAACATGCAGACCGACTAC
GACCTCAAGATCGCCGAGCGTGAACTGAAGGCGAGTTTGCGGAAAGTTCTGCCATTGGCGGCCTGA

Structures

Toxin

• AlphaFold DB: I4WT83