Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 5081733..5082330 Replicon chromosome
Accession NZ_LR890500
Organism Klebsiella pneumoniae isolate KSB1_5H-sc-2280283

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag JMX76_RS25000 Protein ID WP_004142563.1
Coordinates 5081733..5082050 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag JMX76_RS25005 Protein ID WP_004142561.1
Coordinates 5082043..5082330 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX76_RS24985 5077433..5078860 - 1428 WP_009308097.1 MFS transporter -
JMX76_RS24990 5078969..5079835 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
JMX76_RS24995 5080505..5081548 + 1044 WP_040153598.1 DUF2157 domain-containing protein -
JMX76_RS25000 5081733..5082050 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JMX76_RS25005 5082043..5082330 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
JMX76_RS25010 5082595..5083233 - 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
JMX76_RS25015 5083353..5083721 - 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
JMX76_RS25020 5083718..5084302 - 585 WP_094067239.1 TetR/AcrR family transcriptional regulator -
JMX76_RS25025 5084482..5085597 + 1116 WP_012737592.1 MBL fold metallo-hydrolase -
JMX76_RS25030 5085628..5085969 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
JMX76_RS25035 5085987..5086235 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T291210 WP_004142563.1 NZ_LR890500:5081733-5082050 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT291210 WP_004142561.1 NZ_LR890500:5082043-5082330 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References