Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4530977..4531704 Replicon chromosome
Accession NZ_LR890466
Organism Escherichia coli isolate MSB1_7A-sc-2280394

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag JMW16_RS21555 Protein ID WP_000547555.1
Coordinates 4530977..4531288 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JMW16_RS21560 Protein ID WP_000126294.1
Coordinates 4531285..4531704 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW16_RS21530 4526912..4528621 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
JMW16_RS21535 4528631..4529173 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
JMW16_RS21540 4529173..4529940 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
JMW16_RS21545 4529937..4530347 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
JMW16_RS21550 4530340..4530810 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
JMW16_RS21555 4530977..4531288 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
JMW16_RS21560 4531285..4531704 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
JMW16_RS21565 4531783..4533207 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
JMW16_RS21570 4533216..4534673 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
JMW16_RS21575 4534933..4535943 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
JMW16_RS21580 4536092..4536619 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T291110 WP_000547555.1 NZ_LR890466:4530977-4531288 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT291110 WP_000126294.1 NZ_LR890466:4531285-4531704 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References