Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4101031..4101710 Replicon chromosome
Accession NZ_LR890410
Organism Escherichia coli isolate MSB2_1A-sc-2280429

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag JMW04_RS19435 Protein ID WP_000057523.1
Coordinates 4101031..4101333 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag JMW04_RS19440 Protein ID WP_000806442.1
Coordinates 4101369..4101710 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW04_RS19415 4096294..4097514 - 1221 WP_001773866.1 fosmidomycin MFS transporter -
JMW04_RS19420 4097732..4099384 + 1653 WP_001773867.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
JMW04_RS19425 4099421..4099900 - 480 WP_001538397.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
JMW04_RS19430 4100104..4100898 - 795 WP_001538398.1 TraB/GumN family protein -
JMW04_RS19435 4101031..4101333 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JMW04_RS19440 4101369..4101710 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
JMW04_RS19445 4101768..4104272 - 2505 WP_000083948.1 copper-exporting P-type ATPase CopA -
JMW04_RS19450 4104534..4105466 + 933 WP_001538399.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T290933 WP_000057523.1 NZ_LR890410:4101031-4101333 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT290933 WP_000806442.1 NZ_LR890410:4101369-4101710 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References