Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3872450..3873144 Replicon chromosome
Accession NZ_LR890410
Organism Escherichia coli isolate MSB2_1A-sc-2280429

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag JMW04_RS18395 Protein ID WP_001263500.1
Coordinates 3872746..3873144 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JMW04_RS18390 Protein ID WP_000554758.1
Coordinates 3872450..3872743 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW04_RS18365 3868267..3868734 + 468 WP_000725261.1 flagellar basal body-associated FliL family protein -
JMW04_RS18370 3868754..3869470 + 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
JMW04_RS18375 3869483..3870346 + 864 WP_001169518.1 flagellar motor stator protein MotA -
JMW04_RS18380 3870349..3871272 + 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
JMW04_RS18385 3871343..3872398 + 1056 WP_001226168.1 DNA polymerase IV -
JMW04_RS18390 3872450..3872743 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JMW04_RS18395 3872746..3873144 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JMW04_RS18400 3873154..3873606 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
JMW04_RS18405 3873796..3874935 + 1140 WP_000521561.1 RNA ligase RtcB family protein -
JMW04_RS18410 3874932..3875546 + 615 WP_000602124.1 peptide chain release factor H -
JMW04_RS18415 3875603..3877060 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
JMW04_RS18420 3877321..3877779 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T290931 WP_001263500.1 NZ_LR890410:3872746-3873144 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT290931 WP_000554758.1 NZ_LR890410:3872450-3872743 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References