290700

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4399818..4400512 Replicon chromosome
Accession NZ_LR890334
Organism Escherichia coli isolate MINF_9A-sc-2280436

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JMW07_RS21125 Protein ID WP_001263491.1
Coordinates 4400114..4400512 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JMW07_RS21120 Protein ID WP_000554755.1
Coordinates 4399818..4400111 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW07_RS21095 4394854..4395114 - 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -
JMW07_RS21100 4395300..4396073 + 774 WP_022645219.1 C40 family peptidase -
JMW07_RS21105 4396171..4397883 - 1713 Protein_4119 flagellar biosynthesis protein FlhA -
JMW07_RS21110 4397855..4398640 + 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
JMW07_RS21115 4398711..4399766 + 1056 WP_022645222.1 DNA polymerase IV -
JMW07_RS21120 4399818..4400111 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JMW07_RS21125 4400114..4400512 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JMW07_RS21130 4400522..4400974 + 453 WP_023909048.1 GNAT family N-acetyltransferase -
JMW07_RS21135 4401165..4402304 + 1140 WP_022645224.1 RNA ligase RtcB family protein -
JMW07_RS21140 4402301..4402915 + 615 WP_022645225.1 peptide chain release factor H -
JMW07_RS21145 4402977..4403486 + 510 WP_001361775.1 hydrolase -
JMW07_RS21150 4403503..4403784 - 282 WP_022645226.1 hypothetical protein -
JMW07_RS21155 4403793..4405250 - 1458 WP_022645227.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T290700 WP_001263491.1 NZ_LR890334:4400114-4400512 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT290700 WP_000554755.1 NZ_LR890334:4399818-4400111 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References