Detailed information of TA system TA029065

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF1/-
• Location: 1988850..1989157
• Replicon: chromosome
• Accession: NC_020532
• Organism: Staphylococcus aureus subsp. aureus ST228

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: SAI3T3_RS15690
• Protein ID: WP_011447039.1
• Coordinates: 1988981..1989157 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF1
Locus tag	-
Coordinates	1988850..1988989 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAI3T3_RS09840	1984416..1984595	+	180	WP_000669791.1	hypothetical protein	-
SAI3T3_RS14895	1984906..1985166	+	261	WP_001791826.1	hypothetical protein	-
SAI3T3_RS09855	1985219..1985569	-	351	WP_000702263.1	complement inhibitor SCIN-A	-
SAI3T3_RS09860	1986080..1986415	-	336	Protein_1855	SH3 domain-containing protein	-
SAI3T3_RS09865	1987066..1987557	-	492	WP_000920038.1	staphylokinase	-
SAI3T3_RS09870	1987748..1988503	-	756	WP_000861038.1	CHAP domain-containing protein	-
SAI3T3_RS09875	1988515..1988769	-	255	WP_000611512.1	phage holin	-
SAI3T3_RS14900	1988821..1988928	+	108	WP_031762631.1	hypothetical protein	-
-	1988850..1988989	+	140	NuclAT_0	-	Antitoxin
-	1988850..1988989	+	140	NuclAT_0	-	Antitoxin
-	1988850..1988989	+	140	NuclAT_0	-	Antitoxin
-	1988850..1988989	+	140	NuclAT_0	-	Antitoxin
SAI3T3_RS15690	1988981..1989157	-	177	WP_011447039.1	putative holin-like toxin	Toxin
SAI3T3_RS09890	1989266..1990039	-	774	WP_000750412.1	staphylococcal enterotoxin type A	-
SAI3T3_RS09895	1990412..1990786	-	375	WP_000340977.1	hypothetical protein	-
SAI3T3_RS09900	1990842..1991129	-	288	WP_001262621.1	hypothetical protein	-
SAI3T3_RS14905	1991175..1991327	-	153	WP_001000058.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / sak / sea / groEL	1985219..2024750	39531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T29065 WP_011447039.1 NC_020532:c1989157-1988981 [Staphylococcus aureus subsp. aureus ST228]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T29065 NC_020532:c1989157-1988981 [Staphylococcus aureus subsp. aureus ST228]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT29065 NC_020532:1988850-1988989 [Staphylococcus aureus subsp. aureus ST228]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• PDB: 7NS1

Antitoxin