Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3073522..3074124 Replicon chromosome
Accession NZ_LR890299
Organism Escherichia coli isolate MSB1_3B-sc-2280406

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag JM188_RS14685 Protein ID WP_000897305.1
Coordinates 3073813..3074124 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JM188_RS14680 Protein ID WP_000356395.1
Coordinates 3073522..3073812 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JM188_RS14645 3069146..3070048 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
JM188_RS14650 3070045..3070680 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
JM188_RS14655 3070677..3071606 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
JM188_RS14660 3071788..3072030 - 243 WP_001309881.1 ribbon-helix-helix domain-containing protein -
JM188_RS14665 3072249..3072467 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
JM188_RS14670 3072886..3073164 - 279 WP_001315112.1 hypothetical protein -
JM188_RS14675 3073216..3073437 - 222 WP_001550354.1 hypothetical protein -
JM188_RS14680 3073522..3073812 - 291 WP_000356395.1 helix-turn-helix domain-containing protein Antitoxin
JM188_RS14685 3073813..3074124 - 312 WP_000897305.1 hypothetical protein Toxin
JM188_RS14690 3074353..3075261 + 909 WP_001550353.1 alpha/beta hydrolase -
JM188_RS14695 3075429..3077243 - 1815 WP_001550352.1 hypothetical protein -
JM188_RS14700 3077659..3078600 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
JM188_RS14705 3078645..3079082 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T290562 WP_000897305.1 NZ_LR890299:c3074124-3073813 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT290562 WP_000356395.1 NZ_LR890299:c3073812-3073522 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References