290497

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1066722..1067449 Replicon chromosome
Accession NZ_LR890288
Organism Escherichia coli isolate MSB1_1A-sc-2280383

Toxin (Protein)


Gene name higB Uniprot ID A0A829L2T9
Locus tag JMW08_RS05145 Protein ID WP_001521139.1
Coordinates 1066722..1067033 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JMW08_RS05150 Protein ID WP_000126294.1
Coordinates 1067030..1067449 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW08_RS05120 1062657..1064366 + 1710 WP_001521140.1 formate hydrogenlyase subunit HycE -
JMW08_RS05125 1064376..1064918 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
JMW08_RS05130 1064918..1065685 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
JMW08_RS05135 1065682..1066092 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
JMW08_RS05140 1066085..1066555 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
JMW08_RS05145 1066722..1067033 + 312 WP_001521139.1 type II toxin-antitoxin system HigB family toxin Toxin
JMW08_RS05150 1067030..1067449 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
JMW08_RS05155 1067528..1068952 - 1425 WP_020234028.1 6-phospho-beta-glucosidase AscB -
JMW08_RS05160 1068961..1070418 - 1458 WP_020234027.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
JMW08_RS05165 1070678..1071688 + 1011 WP_015912547.1 DNA-binding transcriptional regulator AscG -
JMW08_RS05170 1071837..1072364 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12493.23 Da        Isoelectric Point: 9.4783

>T290497 WP_001521139.1 NZ_LR890288:1066722-1067033 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT290497 WP_000126294.1 NZ_LR890288:1067030-1067449 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L2T9

Antitoxin

Source ID Structure

References