Detailed information of TA system TA290448

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52274..52700
• Replicon: plasmid 2
• Accession: NZ_LR890271
• Organism: Escherichia coli isolate MINF_7C-sc-2280452

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JMW01_RS24290
• Protein ID: WP_001312861.1
• Coordinates: 52274..52432 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	52476..52700 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW01_RS24255	47320..47547	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
JMW01_RS24260	47641..48327	-	687	WP_000332484.1	PAS domain-containing protein	-
JMW01_RS24265	48518..48901	-	384	WP_000124981.1	relaxosome protein TraM	-
JMW01_RS24270	49178..49825	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
JMW01_RS24275	50122..50943	-	822	WP_001234469.1	DUF945 domain-containing protein	-
JMW01_RS24280	51065..51352	-	288	WP_000107535.1	hypothetical protein	-
JMW01_RS24285	51377..51583	-	207	WP_000547939.1	hypothetical protein	-
JMW01_RS24290	52274..52432	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	52476..52700	-	225	NuclAT_0	-	Antitoxin
-	52476..52700	-	225	NuclAT_0	-	Antitoxin
-	52476..52700	-	225	NuclAT_0	-	Antitoxin
-	52476..52700	-	225	NuclAT_0	-	Antitoxin
JMW01_RS24295	52512..52700	+	189	WP_001299721.1	hypothetical protein	-
JMW01_RS24300	52712..53431	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
JMW01_RS24305	53428..53862	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
JMW01_RS24310	53917..55875	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
JMW01_RS24315	55934..56167	-	234	WP_000006004.1	DUF905 domain-containing protein	-
JMW01_RS24320	56223..56750	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aac(3)-IIa / sitABCD / tet(A)	-	1..118421	118421

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T290448 WP_001312861.1 NZ_LR890271:c52432-52274 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT290448 NZ_LR890271:c52700-52476 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin