Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3931526..3932220 Replicon chromosome
Accession NZ_LR890270
Organism Escherichia coli isolate MINF_7C-sc-2280452

Toxin (Protein)


Gene name yafO Uniprot ID V0YKG6
Locus tag JMW01_RS18510 Protein ID WP_001263485.1
Coordinates 3931822..3932220 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JMW01_RS18505 Protein ID WP_000554758.1
Coordinates 3931526..3931819 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMW01_RS18485 3926842..3927339 + 498 WP_000006263.1 REP-associated tyrosine transposase RayT -
JMW01_RS18490 3927879..3929591 - 1713 Protein_3602 flagellar biosynthesis protein FlhA -
JMW01_RS18495 3929563..3930348 + 786 WP_000207554.1 putative lateral flagellar export/assembly protein LafU -
JMW01_RS18500 3930419..3931474 + 1056 WP_001226177.1 DNA polymerase IV -
JMW01_RS18505 3931526..3931819 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JMW01_RS18510 3931822..3932220 + 399 WP_001263485.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JMW01_RS18515 3932230..3932682 + 453 WP_001059899.1 GNAT family N-acetyltransferase -
JMW01_RS18520 3932928..3933134 + 207 Protein_3608 RtcB family protein -
JMW01_RS18525 3933130..3933482 + 353 Protein_3609 peptide chain release factor H -
JMW01_RS18530 3933539..3934996 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JMW01_RS18535 3935257..3935715 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JMW01_RS18540 3935807..3937051 + 1245 WP_000189541.1 esterase FrsA -
- 3936311..3936391 + 81 NuclAT_12 - -
- 3936311..3936391 + 81 NuclAT_12 - -
- 3936311..3936391 + 81 NuclAT_12 - -
- 3936311..3936391 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15418.84 Da        Isoelectric Point: 7.3840

>T290439 WP_001263485.1 NZ_LR890270:3931822-3932220 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT290439 WP_000554758.1 NZ_LR890270:3931526-3931819 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YKG6

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References