Detailed information of TA system TA290420

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 126194..126565
• Replicon: chromosome
• Accession: NZ_LR890270
• Organism: Escherichia coli isolate MINF_7C-sc-2280452

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: JMW01_RS00650
• Protein ID: WP_001317028.1
• Coordinates: 126371..126565 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	126194..126372 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW01_RS00625	122149..123522	+	1374	WP_000123754.1	ATP-dependent RNA helicase DbpA	-
JMW01_RS00630	123651..124586	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
JMW01_RS00635	124638..125873	-	1236	WP_000040858.1	site-specific integrase	-
JMW01_RS00640	125875..126090	-	216	WP_000079604.1	excisionase XisR	-
JMW01_RS00645	126169..126378	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	126194..126372	+	179	NuclAT_0	-	Antitoxin
-	126194..126372	+	179	NuclAT_0	-	Antitoxin
-	126194..126372	+	179	NuclAT_0	-	Antitoxin
-	126194..126372	+	179	NuclAT_0	-	Antitoxin
JMW01_RS00650	126371..126565	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
JMW01_RS00655	126622..127431	-	810	WP_000166319.1	recombination protein RecT	-
JMW01_RS00660	127424..130024	-	2601	WP_001586992.1	exodeoxyribonuclease VIII	-
JMW01_RS00665	130126..130401	-	276	WP_000632297.1	protein RacC	-
JMW01_RS00670	130476..130646	-	171	WP_001352098.1	conserved protein, Rac prophage	-
JMW01_RS00675	130646..130867	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	124638..172191	47553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T290420 WP_001317028.1 NZ_LR890270:c126565-126371 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

Antitoxin

Nucleotide

• Length: 179 bp

>AT290420 NZ_LR890270:126194-126372 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin